Eudicot Evolutionary Research

For Primerdesign and Primer Database management we use the java-based program SeqState. Please find the program and corresponding manual here.
Shown here are only already publicated Primers for different genomic regions, seperated in three groups, one for trnK/matK region,
one for the trnT-L-F region and one for all other currently used regions.

Our database comprises a current (19/10/2007) count of 275 Primers.

Primer Database: – download the SeqState .prs file of publicated (98) Primers here

name	5'->3' sequence	orientation	designed by	used for	target region	publication
atpB-3`F	GTGGAAACCCCGGGACGAGAAGTAGT	F	Hodges and Arnold (1994)	angiosperms	atpB	Hodges and Arnold (1994)
atpb-o12	GAAGTAGTAGGATTGATTCTC	F	Samuel et al (1997)	angiosperms	atpB	Samuel et al (1997)
ITS4	TCCTCCGCTTATTGATATGC	R	White et al (1990)	angiosperms	ITS	White et al (1990)
ITS5	GGAAGTAAAAGTCGTAACAAGG	F	White et al (1990)	angiosperms	ITS	White et al (1990)
ITS5bryo	TCCTCCGCTTAGTGATATGC	F	Quandt (2001)	bryophytes	ITS	Quandt (2001)
HEpetD-343R	TTCTTCTTTAGATCCCTTG	R	Worberg (2005)	internal Helicia	petD	Worberg et al. (2007)
MONpetD738R	AATTTWGCTCTTAATACAGG	R	Löhne and Borsch (2002)	Monimiaceae	petD	Löhne and Borsch (2005)
PIpetB1365F	TTGACYCGTTTTTATAGTTTAC	F	Löhne and Borsch (2002)	angiosperms	petD	Löhne and Borsch (2005)
PIpetB1411F	GCCGTMTTTATGTTAATGC	F	Löhne and Borsch (2002)	angiosperms	petD	Löhne and Borsch (2005)
PIpetD346R	TCTTCCTYAGATCCC	R	Löhne and Borsch (2002)	angiosperms	petD	Löhne and Borsch (2005)
PIpetD738R	AATTTAGCYCTTAATACAGG	R	Löhne and Borsch (2002)	angiosperms	petD	Löhne and Borsch (2005)
rbcL-1460R	CTTTTAGTAAAAGATTGGGCCGAG	R	Chase et al (1993)	angiosperms	rbcL	Chase et al (1993)
rbcL-1F	ATGTCACCACAAACAGAAACTAAAGC	F	Chase et al (1993)	angiosperms	rbcL	Chase et al (1993)
rbcL-o15	TACAGTTGTCCATGTACCAG	F	Olmstead	angiosperms	rbcL	Olmstead
rpl16F	CTATGCTTAGTGTGTGACTC	F	Campagna and Downie (1998)	angiosperms	rpl16	Campagna and Downie (1998)
rpl16R	TCTTCCTCTATGTTGTTTACG	R	Campagna and Downie (1998)	angiosperms	rpl16	Campagna and Downie (1998)
Primers for the trnK/matK region
ACmatK100F	CTCGACTGTATCAACAGAATC	F	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK105F	GAATGAATGGAAAAAACAGCATG	F	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK1200R	AGGTATTAATATATCTAACAC	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK1250F	CTCATTATTATAGTGGCTCYTC	F	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK1252R	TGAGAATTGATCCTCTAAAAAAGG	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
Acmatk1300R	TRCGAGACAAAGTTCTAGCAG	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK1400R	GGATTCGTATTCACATACA	R	Quandt (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK1426R	AAAGGAAATGGATTCTTTTGG	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK1497R	CATCTTTCACCCAGGGAAG	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK1530R	ATAAAGAAAGAATCGTAATAAATG	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK1746F	CTTCAAAAGGGACATCTCTTCTG	F	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK1809F	AATGTTATTTTTCTGTGTGGTCTC	F	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK1860R	GAATCGCACACTTGAAAGAAAACC	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK1983F	TAAAAAATTCGATACCATAGTTCC	F	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK200R	GAATTTCCACAATGACTGC	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK2080R	GTCCAAATCGGTCAATAATATC	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK2113F	TCAGTAATACGGAGTCAAATG	F	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK2231R	TCTTCCAAAAATTCTGAACCT	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK500F	TTCTTCTTTGCATTTATTACG	F	Kai Mueller (2002)	Amaranthaceae	matK	Hilu et al. (2003)
ACmatK634R	ATAGGAACAAGAATAATCT	R	Kai Mueller (2001)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatk650r	GGATTCATATTCACATACATRG	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatk700R	TCCGTAGAAATGKATTCGTTC	R	Quandt (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK871R	TATTCCTGAAAGATAAGTGGATAG	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK873R	ATATACTCCTGAAAGAGAAGTGG	R	Kai Mueller (2002)	Amaranthaceae	matK	Müller and Borsch (2005)
ACmatK975F	ATACAGTTTACTAATWGTRAAACG	F	Wanke (2001)	Amaranthaceae	matK	Müller and Borsch (2005)
ALImatK470F	CCSTATCCCATTCATCTCG	F	Wanke (2001)	Alismataceae	matK	Hilu et al. (2003)
ARAmatK480F	CATCTCGAAATCTTGGTTC	F	Wanke (2001)	Araceae	matK	Hilu et al. (2003)
DIDYmatK-1035R	CGAACTCGTAAAGACTCGA	R	Worberg (2005)	Didymeles	matK	Worberg et al. (2007)
DIDYmatK-1107F	CAATTACTCCTCTGATTGGATC	F	Worberg (2005)	Didymeles internal	matK	Worberg et al. (2007)
DIDYmatk-570F	CGAGTATCAGAACTGGAG	F	Worberg (2005)	Didymeles	matK	Worberg et al. (2007)
EUPTmatK-1006F	GGCTATCTTTCAAGTGTACG	F	Worberg (2005)	Euptelea	matK	Worberg et al. (2007)
GNmatK1056R	TCCCTGGTAATGAACTCTGC	R	Borsch (2001)	Gnetales	matK	Hilu et al. (2003)
Le1R	ATAGAAATAGATTCGTTC	R	Kai Mueller (2001)	Lentibulariaceae	matK	Müller et al. (2004)
Le2F	TGGTACGGAGTCAAAKTC	F	Kai Mueller (2001)	Lentibulariaceae	matK	Müller et al. (2004)
Le3F	ACCAATCCTTTCATTTACG	F	Kai Mueller (2001)	Lentibulariaceae	matK	Müller et al. (2004)
Le4R	TTCGCCTGAAAATCCGTAACC	R	Kai Mueller (2001)	Lentibulariaceae	matK	Müller et al. (2004)
Le7F	GGGTTGCTAACTCAACGGTAG	F	Kai Mueller (2001)	Lentibulariaceae	matK	Müller et al. (2004)
MATKFA490F	GAAATCTTGGTTCAAAYCCTTCG	F	Kai Mueller (2001)	angiosperms	matK	Hilu et al. (2003)
MATKGE490F	ATCTTGGTTCAAACYCTTCGC	F	Kai Mueller (2001)	angiosperms	matK	Hilu et al. (2003)
MG1	AACTAGTCGGATGGAGTAGAT	R	Liang and Hilu (1996)	angiosperms	matK	Liang and Hilu (1996)
MG15	ATCTGGGTTGCTAACTCAATG	F	Liang and Hilu (1996)	angiosperms	matK	Liang and Hilu (1996)
NYmatK_180R	TAAGCGTTTCACAATTAGTG	R	Borsch (2000)	Nymphaeales	matK	Borsch (2000)
NYmatK480F	CATCTGGAAATCTTGSTTC	F	Borsch (2000)	Nymphaeales	matK	Borsch (2000)
PI-matK-1060F	ACTTRTGGTCTCAAC	F	Wanke and Borsch (2001)	Piperaceae	matK	Wanke et al. (2006)
PI-matK-1480F	TCGTAAACAYAAAAGTAC	F	Wanke and Borsch (2001)	Piperaceae	matK	Wanke et al. (2006)
PI-matK-370R	TTTYCCTATAATTGGAGC	R	Wanke and Borsch (2001)	Piperaceae	matK	Wanke et al. (2006)
Pi-matK-470F	TTCAAACCCTTCGYTRCTGG	F	Wanke (2001)	Piperaceae	matK	Hilu et al. (2003)
PI-matK-500F	TTTGCATTTATTGCGAATC	F	Wanke and Borsch (2001)	Piperaceae	matK	Wanke et al. (2006)
PI-matK-560F	TGGATACAAGATGTTCCC	F	Wanke and Borsch (2001)	Piperaceae	matK	Wanke et al. (2006)
PI-matK-700R	ATRAGAAGATTGTTTACGG	R	Wanke and Borsch (2001)	Piperaceae	matK	Wanke et al. (2006)
PI-matK-730R	ATAGAAATGGAYTCGTTCAAG	R	Wanke and Borsch (2001)	Piperaceae	matK	Wanke et al. (2006)
PRmatK521F	TTCYTTGCATTTATTGCGATTC	F	Borsch (2001)	angiosperms	matK	Hilu et al. (2003)
psbA-R	CGCGTCTCTCTAAAATTGCAGTCAT	R	Steele and Vilgalys (1994)	angiosperms	matK	Steele (1995)
RAmatK470F	ACCCTACCCYATCCATCTG	F	Kai Mueller (2001)	angiosperms	matK	Hilu et al. (2003)
RAmatK670F	CCRTATTTGTTTTTCTCCG	F	Borsch (2001)	angiosperms	matK	Hilu et al. (2003)
ROSmatK-530F	AGATGCCTCTTCTTTGC	F	Worberg (2006)	rosid matK internal	matK	Worberg et al. (2007)
ROSmatK-655R	GGATTCGTATTCACATACAT	R	Worberg (2006)	rosid matK internal	matK	Worberg et al. (2007)
TOmatK480F	CATCTKGAAATCTTGGTTC	F	Borsch (2001)	angiosperms	matK	Hilu et al. (2003)
Wel-matK	TACTTAATACGATCGAAATATGC	F	Wanke and Borsch (2001)	Welwitschia	matK	Hilu et al. (2003)
Za-matK	AATGGAAGATGCTTCTCC	F	Wanke and Borsch (2001)	Zamia	matK	Hilu et al. (2003)
trnK1280R	ATAAAAGCAAACCCCTCTG	R	Kai Mueller (2001)	angiosperms	trnK	Müller et al. (2004)
trnK1280RA	ATAACAGCAAACCCTTCTG	R	Kai Mueller (2001)	angiosperms	trnK	Müller et al. (2004)
trnK1280RB	ATACAAACAAAAACCTCTG	R	Kai Mueller (2001)	angiosperms	trnK	Müller et al. (2004)
trnK1580F	TACGATTCTTTCTCAGCG	F	Kai Mueller (2001)	angiosperms	trnK	Müller et al. (2004)
trnK1580FC	TACGATTCTTGCTCAACG	F	Kai Mueller (2001)	angiosperms	trnK	Müller et al. (2004)
trnK1590R	CTCGCTGAGAAAGAATCG	R	Kai Mueller (2001)	angiosperms	trnK	Müller et al. (2004)
trnK1800F	ACGATCAACATCTTCTGGAG	F	Kai Mueller (2001)	angiosperms	trnK	Müller et al. (2004)
trnK1800R	AAATGAAAGGAGTGGTTGC	R	Kai Mueller (2001)	angiosperms	trnK	Müller et al. (2004)
trnK2000F	CCTGAATAAATGGAAATG	F	Kai Mueller (2001)	angiosperms	trnK	Müller et al. (2004)
trnK2050F	TTTTCCTTGTGGTTTCCTG	F	Kai Mueller (2001)	angiosperms	trnK	Müller et al. (2004)
trnK2450R	CTTTGTGTTTCCGAGCC	R	Kai Mueller (2001)	angiosperms	trnK	Müller et al. (2004)
trnK2600F	GTCGGATTTGGTATTTGG	F	Kai Mueller (2001)	angiosperms	trnK	Müller et al. (2004)
trnK2R	AACTAGTCGGATGGAGTAG	R	Johnson and Soltis (1995)	angiosperms	trnK	Johnson and Soltis (1995)
*Primers for the trnT-L-F* region**
trnL-110R	GATTTGGCTCAGGATTGCCC	R	Wanke and Borsch (2001)	angiosperms	trnT-F	Borsch et al. (2003)
trnL-460F	GAGAATAAAGATAGAGTCC	F	Worberg (2005)	angiosperms	trnT-F	Worberg et al. (2007)
trnTa	CATTACAAATGCGATGCTCT	F	Taberlet et al (1991)	angiosperms	trnT-F	Taberlet et al (1991)
trnTB	TCTACCGATTTCGCCATATC	R	Taberlet et al (1991)	angiosperms	trnT-F	Taberlet et al (1991)
trnTc	CGAAATCGGTAGACGCTACG	F	Taberlet et al (1991)	angiosperms	trnT-F	Taberlet et al (1991)
trnTd	GGGGATAGAGGGACTTGAAC	R	Taberlet et al (1991)	angiosperms	trnT-F	Taberlet et al (1991)
trnTe	GGTTCAAGTCCCTCTATCCC	F	Taberlet et al (1991)	angiosperms	trnT-F	Taberlet et al (1991)
trnTf	ATTTGAACTGGTGACACGAG	R	Taberlet et al (1991)	angiosperms	trnT-F	Taberlet et al (1991)